Difference between revisions of "Part:BBa K1129037"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1129037 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1129037 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1129037 parameters</partinfo>
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  The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.  
 
  The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.  
  
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'''Resources'''
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Resources'''
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http://www.uniprot.org/uniprot/Q1H8P3
 
http://www.uniprot.org/uniprot/Q1H8P3
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http://www.genome.jp/kegg-bin/show_pathway?map00940
 
http://www.genome.jp/kegg-bin/show_pathway?map00940
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===Functional Parameters===
 
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<partinfo>BBa_K1129037 parameters</partinfo>
 
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Revision as of 03:08, 28 September 2013

4-coumarate-CoA ligase (4CL) under arabinose promoter

Arab.+rbs+4-CL+term.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1242
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1809
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1441



The 4-coumarate-CoA ligase (4CL) enzyme catalyzes the ligation of a CoA group to the end of the molecule. The addition of this CoA provides better kinetics for a variety of downstream reactions. 4CL is also a member of the phenylpropanoid pathway and is found in a wide variety of organisms that partake in the degradation of lignin. Here we harness the 4CL to provide better kinetics for reduction of cinnamic acid to cinnamaldehyde.

Resources

http://www.uniprot.org/uniprot/Q1H8P3

http://en.wikipedia.org/wiki/4-Coumarate-CoA_ligase

http://www.genome.jp/kegg-bin/show_pathway?map00940