Difference between revisions of "Part:BBa K1162006"
Ryanputman (Talk | contribs) |
Ryanputman (Talk | contribs) |
||
| Line 8: | Line 8: | ||
This part was codon optimized for <i>E. coli</i> using the Life Technologies GeneArt ® software program. By design, this part is [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] and contains a Methionine(atg) residue at the start of the coding region. Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction. | This part was codon optimized for <i>E. coli</i> using the Life Technologies GeneArt ® software program. By design, this part is [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] and contains a Methionine(atg) residue at the start of the coding region. Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction. | ||
| − | === | + | ===Improvement on Existing Parts (as of September 2013)=== |
| − | + | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114] Slovenia iGEM team 2009 - lacks a start codon. | |
| − | + | ||
Previously this part has been used Trieste iGEM team 2012 | Previously this part has been used Trieste iGEM team 2012 | ||
https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 | https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 | ||
This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification. Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag). | This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification. Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag). | ||
| + | |||
| + | ===References=== | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 02:09, 28 September 2013
LL-37 antimicrobial peptide from Humans (Homo sapiens)
LL-37 is an antimicrobial peptide (AMP) found in humans.
Design
This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region. Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction.
Improvement on Existing Parts (as of September 2013)
BBa_K245114 Slovenia iGEM team 2009 - lacks a start codon. Previously this part has been used Trieste iGEM team 2012 https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification. Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag).
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]