Difference between revisions of "Part:BBa K1021020:Design"

 
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===References===
 
===References===
 +
[https://parts.igem.org/Part:BBa_I712074 1. T7 promoter part page]
 +
<br>
 +
[https://parts.igem.org/Part:BBa_K082003?title=Part:BBa_K082003 2. GFP+LVA part page]

Latest revision as of 02:06, 28 September 2013


PT7+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


Design Notes

This reporter does not contain a bacterial RBS and is therefore suited for T7-based expression in eukaryotes.


Source

The promoter originates from the T7 virus and the gene originates from the vector pHTSUB-105.

References

1. T7 promoter part page
2. GFP+LVA part page