Difference between revisions of "Part:BBa K1162006"
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<partinfo>BBa_K1162006 short</partinfo>
 
<partinfo>BBa_K1162006 short</partinfo>
  
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This is an antimicrobial peptide (AMP) is commonly found in humans. This part was commercially synthesized and codon optimized for expression in E. coli and is an improvement on LL-37 parts currently in the registry. The prefix and suffix are compatible with RFC 23 (silver fusion) to allow for protein fusions and this part contains an ‘atg’ to allow for immediate protein synthesis.  Furthermore this LL-37 derivative does not have any stop codons which allows for composite BioBrick  
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LL-37 is an antimicrobial peptide (AMP) found in humans.
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Related parts
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===Design===
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This part was codon optimized for <i>E. coli</i> using the Life Technologies GeneArt &#174; software program. By design, this part is [https://parts.igem.org/Assembly_standard_23 Assembly Standard #23 (Silver Fusion)] and contains a Methionine(atg) residue at the start of the coding region.  Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction.
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===Related parts===
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Previously this part has been used by Slovenia iGEM team 2009 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K245114)
 
Previously this part has been used by Slovenia iGEM team 2009 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K245114)
 
This part (BBa_K245114) lacks a start codon.
 
This part (BBa_K245114) lacks a start codon.

Revision as of 02:01, 28 September 2013

LL-37 antimicrobial peptide from Humans (Homo sapiens)

LL-37 is an antimicrobial peptide (AMP) found in humans.

Design

This part was codon optimized for E. coli using the Life Technologies GeneArt ® software program. By design, this part is Assembly Standard #23 (Silver Fusion) and contains a Methionine(atg) residue at the start of the coding region. Furthermore, this LL-37 sequence does not have any stop codons, which allows for composite BioBrick construction.

Related parts

Previously this part has been used by Slovenia iGEM team 2009 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K245114) This part (BBa_K245114) lacks a start codon. Previously this part has been used Trieste iGEM team 2012 https://parts.igem.org/Part:BBa_K875009?title=Part:BBa_K875009 This part, BBa_K875009, has a BioBrick prefix and suffix of RFC 10 so cannot be used for protein fusions, such as in cases of secretion and protein purification. Also has a stop codon which limits what can be added to the C-terminal end (purification tag/secretion tag).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]