Difference between revisions of "Part:BBa K1021033:Design"

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===References===
 
===References===
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[http://www.pnas.org/content/91/26/12649.full.pdf Tagged mutations at the Toxl locus of <i>Cochliobolus heterostrophus</i> by restriction enzyme-mediated integration]
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[http://www.pnas.org/content/91/26/12649.full.pdf [1] Tagged mutations at the Toxl locus of <i>Cochliobolus heterostrophus</i> by restriction enzyme-mediated integration]
 
<br>
 
<br>
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[http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pUCATPH/ pUCATPH sequence on SnapGene]
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[http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pUCATPH/ [2] pUCATPH sequence on SnapGene]

Revision as of 02:00, 28 September 2013


PT7+hph


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 391
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As this part was not designed for bacterial expression, it does not contain a bacterial RBS.


Source

The promoter comes from the T7 virus; hph comes from E. coli and was taken from the pUCATPH transformation vector.

References

[http://www.pnas.org/content/91/26/12649.full.pdf [1] Tagged mutations at the Toxl locus of Cochliobolus heterostrophus by restriction enzyme-mediated integration]
[http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pUCATPH/ [2] pUCATPH sequence on SnapGene]