Difference between revisions of "Part:BBa K1195003"

Revision as of 01:04, 28 September 2013

Promoter Qrr4 + RFP

In Vibrio cholerae the Qrr4 gene is part of the quorum sensing genes. When Vibrio cholerae is not present it controls the formation of a sRNA which affects the virulence and biofilm formation of V.cholerae. We attached the promoter for Qrr4 to the reporter protein RFP to help us detect the presence of V.cholerae.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 741
Illegal AgeI site found at 853
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1195003 short</partinfo>
-The promoter Qrr4 attached to the reporter protein RFP that has a hard terminator added.
+In Vibrio cholerae the Qrr4 gene is part of the quorum sensing genes. When Vibrio cholerae is not present it controls the formation of a sRNA which affects the virulence and biofilm formation of V.cholerae. We attached the promoter for Qrr4 to the reporter protein RFP to help us detect the presence of V.cholerae.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part is intended to be used in the presence of other quorum sensing genes from V.cholerae that have been transformed into E.coli.
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