Difference between revisions of "Part:BBa K1100110"
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| − | + | We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR. The following chart shows the distribution of sfGFP expression in the circumstance of promoter J23110. | |
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| + | [[File:pSB1C3-J23110-f.jpg|500px|thumb|center|'''Figure 1'''. The distribution of the sfGFP expression tested by flow cytometry with promoter J23110 and Csy4 insulator.]] | ||
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| + | [[File:fc--c.jpg|400px|thumb|center|'''Figure 2'''. The intensity of different promoters is shown by the column height and the randomness of their functions by the bar width.]] | ||
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Latest revision as of 23:59, 27 September 2013
J23110 with insulator and sfGFP
We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR. The following chart shows the distribution of sfGFP expression in the circumstance of promoter J23110.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]