Difference between revisions of "Part:BBa R0063:Experience"

Revision as of 23:42, 27 September 2013

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Applications of BBa_R0063

The stochastic dynamics of the constitutive promoter family by 2013 Fudan iGEM team

We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR. The following chart shows the distribution of sfGFP expression in the circumstance of promoter R0063.

Figure 1. The distribution of the sfGFP expression tested by flow cytometry with promoter R0063 and Csy4 insulator.

Figure 2. The intensity of different promoters is shown by the column height and the randomness of their functions by the bar width.

User Reviews

UNIQ2e9ba0ef68aad50e-partinfo-00000000-QINU

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

UNIQ2e9ba0ef68aad50e-partinfo-00000002-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_R0063===
+====The stochastic dynamics of the constitutive promoter family by 2013 Fudan iGEM team====
+We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR. The following chart shows the distribution of sfGFP expression in the circumstance of promoter R0063.
+[[File:pSB1C3-R0063-f.jpg|500px|thumb|center|'''Figure 1'''. The distribution of the sfGFP expression tested by flow cytometry with promoter R0063 and Csy4 insulator.]]
+[[File:fc--c.jpg|400px|thumb|center|'''Figure 2'''. The intensity of different promoters is shown by the column height and the randomness of their functions by the bar width.]]
 ===User Reviews===