Difference between revisions of "Part:BBa K1114211"

Latest revision as of 23:26, 27 September 2013

E1010m_CD

This is a MoClo formatted E1010 RFP gene with CD fusion sites. This is a modified BBa_E1010 converted to Moclo with one cut site eliminated in the middle of the sequence.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Source

iGEM distribution kit 2013

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 559
Illegal AgeI site found at 671
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1114211 short</partinfo>
-Modified BBa_E1010 converted to Moclo with one cut site eliminated in the middle of the sequence.
+<html>
+This is a <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted <a href="https://parts.igem.org/Part:BBa_E1010">E1010 RFP</a> gene with CD fusion sites. This is a modified BBa_E1010 converted to Moclo with one cut site eliminated in the middle of the sequence.
 <br><br>
-The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
+This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
+The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
+<br><br>
+</html>
+===Source===
+iGEM distribution kit 2013
 <!-- Add more about the biology of this part here