Difference between revisions of "Part:BBa K1114210"

 
 
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<partinfo>BBa_K1114210 short</partinfo>
 
<partinfo>BBa_K1114210 short</partinfo>
  
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RFP Reporter E1010 in Moclo form
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This is a <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted <a href="https://parts.igem.org/Part:BBa_E1010">E1010 RFP</a> gene with CD fusion sites.
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<br><br>
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This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
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The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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</html>
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===Source===
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iGEM distribution kit 2013
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:25, 27 September 2013

E1010_CD


This is a MoClo formatted E1010 RFP gene with CD fusion sites.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Source

iGEM distribution kit 2013


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 559
    Illegal AgeI site found at 671
  • 1000
    COMPATIBLE WITH RFC[1000]