Difference between revisions of "Part:BBa K1114207"

Latest revision as of 23:22, 27 September 2013

CyPet_CD

This is a MoClo formatted CyPet fluorescent reporter gene with CD fusion sites.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Usage and Biology

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 218
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 218
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 218
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 648

Source

AddGene plasmid #14030; derived from FRET proteins

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1114207 short</partinfo>
-Cyan Fluorescent Protein in Moclo form
+<html>
+This is a <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted CyPet fluorescent reporter gene with CD fusion sites.
-<!-- Add more about the biology of this part here
+<br><br>
+This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
+The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
+<br><br>
+</html>
 ===Usage and Biology===
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1114207 SequenceAndFeatures</partinfo>
+===Source===
+AddGene plasmid #14030; derived from FRET proteins
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
 <partinfo>BBa_K1114207 parameters</partinfo>
-<!-- -->