Difference between revisions of "Part:BBa K1114206"

 
 
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<partinfo>BBa_K1114206 short</partinfo>
 
<partinfo>BBa_K1114206 short</partinfo>
  
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CviR_CD is moclo version of CviR of the CviR/CviI system, analogues of LuxI and R system
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This is a <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted CviR gene with CD fusion sites. This is a LuxR-homologue. CviR is the repressor gene in quorum sensing in <i>Chromobacterium violaceum</i>.
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<br><br>
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This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
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The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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===Source===
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Chromobacterium violaceum strain ATCC 12472 genomic DNA, obtained from the Holden Lab at the University of Wisconsin-Madison
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1114206 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1114206 SequenceAndFeatures</partinfo>

Latest revision as of 23:20, 27 September 2013

CviR_CD


This is a MoClo formatted CviR gene with CD fusion sites. This is a LuxR-homologue. CviR is the repressor gene in quorum sensing in Chromobacterium violaceum.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Source

Chromobacterium violaceum strain ATCC 12472 genomic DNA, obtained from the Holden Lab at the University of Wisconsin-Madison

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 44
    Illegal NgoMIV site found at 571
    Illegal AgeI site found at 325
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 338