Difference between revisions of "Part:BBa K1114202"
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<partinfo>BBa_K1114202 short</partinfo>
 
<partinfo>BBa_K1114202 short</partinfo>
  
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Moclo analogue of bba_c0040 with C and D fusion sites added
 
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===Applications of BBa_K1114201===
 
 
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_C0040 with CD fusion sites. See the <a href="https://parts.igem.org/Part:BBa_C0040">BBa_C0040</a> page for full information on the part.  
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This is a <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted <a href="https://parts.igem.org/Part:BBa_C0012">C0012 lacI</a> gene with CD fusion sites.  
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<br><br>
 
This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
 
This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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Summary of modifications from original part:
 
  
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href="https://parts.igem.org/Part:pSB1C3?title=Part:pSB1C3">pSB1C3</a>.
 
 
</html>
 
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K1114202 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1114202 SequenceAndFeatures</partinfo>
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===Source===
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iGEM distribution kit 2013
  
  
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===Functional Parameters===
 
 
<partinfo>BBa_K1114202 parameters</partinfo>
 
<partinfo>BBa_K1114202 parameters</partinfo>
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Revision as of 23:14, 27 September 2013

C0040_CD, Moclo of BBA_C0040

This is a MoClo formatted C0012 lacI gene with CD fusion sites.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Usage and Biology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

iGEM distribution kit 2013