Difference between revisions of "Part:BBa K1114200"
 
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This is a <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted spectinimycin resistance gene with CD fusion sites.  
 
This is a <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted spectinimycin resistance gene with CD fusion sites.  
  
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This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
 
This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.

Latest revision as of 23:11, 27 September 2013

aaDa_CD

This is a MoClo formatted spectinimycin resistance gene with CD fusion sites.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Usage and Biology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Genomic DNA from E. coli K12 MG1655