Difference between revisions of "Part:BBa K1195000:Design"
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===Design Notes===
 
===Design Notes===
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No specific design considerations were needed.  
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We designed primers to remove the signal sequence from this nucleotide sequence to allow for intracellular expression of the protein.
  
  
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===Source===
 
===Source===
  
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iGem part BBa_K802001 received from the Lyon-INSA iGem team. We also amplified the gene from ''Actinobacilus Pleuropneumoniae''.
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We amplified the Dispersin B gene from iGem part BBa_K802001 received from the Lyon-INSA iGem team. Although Dispersin B had several entries in the iGEM registry, no physical part had been submitted until our submission.
  
 
===References===
 
===References===

Latest revision as of 23:07, 27 September 2013

Dispersin B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 970
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed primers to remove the signal sequence from this nucleotide sequence to allow for intracellular expression of the protein.


Source

We amplified the Dispersin B gene from iGem part BBa_K802001 received from the Lyon-INSA iGem team. Although Dispersin B had several entries in the iGEM registry, no physical part had been submitted until our submission.

References

Pitts, B.; Hamilton, M.; Zelver, N.; Stewart, P. A Microtiter-Plate Screening Method for Biofilm Disinfection and Removal. J. Micro Methods 2003, 54, 269-276.

Kaplan, J. Therapeutic Potential of Biofilm-Dispersing Enzymes. Int. J. Artif. Organs 2009, 32, 545-554.

Ragunath, C.; Shanmugam, M.; Bendaoud, M.; Kaplan, J.; Ramasubbu, N. Effect of a Biofilm-Degrading Enzyme from an Oral Pathogen in Transgenic Tobacco on the Pathogenicity of Pectobacterium Carotovorum Subsp Carotovorum. Plant Pathol. 2012, 61, 346-354.

Gokcen, A.; Vilcinskas, A.; Wiesner, J. Methods to Identify Enzymes that Degrade the Main Extracellular Polysaccharide Component of Staphylococcus epidermidis Biofilms. Virulence 2013, 4, 260-270.