Difference between revisions of "Part:BBa K1132037"
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<partinfo>BBa_K1132037 short</partinfo> | <partinfo>BBa_K1132037 short</partinfo> | ||
| − | This BioBrick is design to test the AND gate (BBa_K1132034) by measuring the level of RFP after recombination events. The biobrick | + | This BioBrick is design to test the AND gate ([https://parts.igem.org/Part:BBa_K1132034 BBa_K1132034]) by measuring the level of RFP after recombination events. The biobrick [https://parts.igem.org/Part:BBa_K081014 BBa_K081014] containing the RBS site, the coding sequence of the RFP and a terminator have been inserted inside our gate between the FimE restrictions sites. The T7 polymerase gene is also present in the biobrick, under the control of a strong promoter, strong RBS. This part can therefore be used stand-alone as all elements to control the RFP output are present. |
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Revision as of 23:05, 27 September 2013
AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter
This BioBrick is design to test the AND gate (BBa_K1132034) by measuring the level of RFP after recombination events. The biobrick BBa_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been inserted inside our gate between the FimE restrictions sites. The T7 polymerase gene is also present in the biobrick, under the control of a strong promoter, strong RBS. This part can therefore be used stand-alone as all elements to control the RFP output are present.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1141
Illegal NheI site found at 1164 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 957
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 264
Illegal AgeI site found at 376 - 1000COMPATIBLE WITH RFC[1000]