Difference between revisions of "Part:BBa K1051280"

 
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__NOTOC__
 
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<partinfo>BBa_K1051280 short</partinfo>
 
<partinfo>BBa_K1051280 short</partinfo>
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<h3>Purpose</h3>
 
Measurement parts of PEST of cln3.
 
Measurement parts of PEST of cln3.
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<br />
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<h3>Principle</h3>
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'''PEST''',Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.
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<br />
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<h3>Engineering</h3>
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We convet base "A" to "G" at 247 site of cln3-PEST(K1051204) by designing primer.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<h3>Sequence and Features</h3>
 
<partinfo>BBa_K1051280 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1051280 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K1051280 parameters</partinfo>
 
<partinfo>BBa_K1051280 parameters</partinfo>
 
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<h3>References</h3>
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[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975. <br />
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[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".

Revision as of 22:57, 27 September 2013

Measurement parts of PEST of cln3.

Purpose

Measurement parts of PEST of cln3.

Principle

PEST,Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.

Engineering

We convet base "A" to "G" at 247 site of cln3-PEST(K1051204) by designing primer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199


References

[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975.
[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".