Difference between revisions of "Part:BBa K1051281"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1051281 short</partinfo>
 
<partinfo>BBa_K1051281 short</partinfo>
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<h3>Purpose</h3>
 
Measurement parts of PEST of cln2.
 
Measurement parts of PEST of cln2.
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<br />
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<h3>Principle</h3>
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'''PEST''',Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.
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<br />
  
 
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<span class='h3bb'>Sequence and Features</span>
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<h3>Sequence and Features</h3>
 
<partinfo>BBa_K1051281 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1051281 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K1051281 parameters</partinfo>
 
<partinfo>BBa_K1051281 parameters</partinfo>
 
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<h3>References</h3>
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[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975. <br />
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[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".

Latest revision as of 22:53, 27 September 2013

Measurement parts of PEST of cln2.

Purpose

Measurement parts of PEST of cln2.

Principle

PEST,Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199


References

[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975.
[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".