Difference between revisions of "Part:BBa K1051278"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1051278 short</partinfo>
 
<partinfo>BBa_K1051278 short</partinfo>
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<h3>Purpose</h3>
 
The measurement of PEST of cln1.
 
The measurement of PEST of cln1.
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<h3>Principle</h3>
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'''PEST''',Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.
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<br />
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<h3>Engineering</h3>
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We convet base "A" to "G" at 348 site of cln1-PEST(K1051202) by overlap PCR.
  
 
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<partinfo>BBa_K1051278 parameters</partinfo>
 
<partinfo>BBa_K1051278 parameters</partinfo>
 
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<h3>References</h3>
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[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975. <br />
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[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".

Revision as of 22:51, 27 September 2013

The measurement of PEST of cln1.

Purpose

The measurement of PEST of cln1.

Principle

PEST,Certain amino acid sequences appear to be signals for degradation. One such sequence is known as the PEST sequence because short stretch of about eight amino acids is enriched with proline(P), glutamic acid(E), serine(S), and threonine(T) at C-terminal of proteins that are more rapidly degraded than other proteins.

Engineering

We convet base "A" to "G" at 348 site of cln1-PEST(K1051202) by overlap PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199


References

[1] Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C, Huang CC, Kain SR(1998) Generation of destabilized green fluorescent protein as a transcription reporter. J Biol Chem 273:34970–34975.
[2] Dorota Skowyra, Karen L Craig, Mike Tyers, Stephen J Elledge, J.Wade Harper "F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex".