Difference between revisions of "Part:BBa K1189005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We designed primers that would produce these TALE | + | We designed primers that would produce these TALE B target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone. |
| + | We expected a sequence of AGCAATGGG in the repeat variable di-residue of the second repeat of TALB, however, the sequence was actually TCCCACGAC. This meant that the required target sequence at this position was a C, and not a T, as the parts registry web page indicates. Therefore, this part has the updated TALB target sequence with C instead of the T. | ||
| Line 12: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | The target sequence was amplified with primers from the genome of <i>E. coli</i> using polymerase chain reaction. | |
===References=== | ===References=== | ||
Revision as of 22:51, 27 September 2013
TALEB Target ([B])
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 213
Illegal AgeI site found at 325 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed primers that would produce these TALE B target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone. We expected a sequence of AGCAATGGG in the repeat variable di-residue of the second repeat of TALB, however, the sequence was actually TCCCACGAC. This meant that the required target sequence at this position was a C, and not a T, as the parts registry web page indicates. Therefore, this part has the updated TALB target sequence with C instead of the T.
Source
The target sequence was amplified with primers from the genome of E. coli using polymerase chain reaction.