Difference between revisions of "Part:BBa K1189004:Design"
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
Primers
+
The target sequence was amplified with primers from the genome of <i>E. coli</i> using polymerase chain reaction.
  
 
===References===
 
===References===

Revision as of 22:48, 27 September 2013

TALEA Target ([A])


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 213
    Illegal AgeI site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed primers that would produce these TALE A target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone.

Source

The target sequence was amplified with primers from the genome of E. coli using polymerase chain reaction.

References