Difference between revisions of "Part:BBa K1080010"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1080010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1080010 SequenceAndFeatures</partinfo>
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<br>
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<br>
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<br>
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<b>Amino acid sequence</b>
  
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<FONT FACE="courier">MAPAAASADK ATAAEYYALV CNAEWFFMDP QNESVAEQLR EKVRFFKEQN KERDFFIVPN<br> PKWLDAKFPE QAKQVKRPCV ALVSTDKMWI TFMKLRLDRV LKIDLKSMPA SEVLAAGEAL<br> PDFKPDGKWT APYARYTPGW WNVFLPNH </F> 
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 +
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References and documentation are available.
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Please note the modified algorithm for extinction coefficient.
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 +
--------------------------------------------------------------------------------
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Number of amino acids: 148
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Molecular weight: 17073.7
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Theoretical pI: 8.50
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Amino acid composition: Ala (A)  20 13.5%
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Arg (R)  7   4.7%
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Asn (N)  6   4.1%
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Asp (D)  9   6.1%
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Cys (C)  2   1.4%
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Gln (Q)  5   3.4%
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Glu (E)  10   6.8%
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Gly (G)  3   2.0%
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His (H)  1   0.7%
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Ile (I)  3   2.0%
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Leu (L)  11   7.4%
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Lys (K)  14   9.5%
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Met (M)  5   3.4%
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Phe (F)  10   6.8%
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Pro (P)  12   8.1%
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Ser (S)  5   3.4%
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Thr (T)  5   3.4%
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Trp (W)  6   4.1%
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Tyr (Y)  4   2.7%
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Val (V)  10   6.8%
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Pyl (O)  0   0.0%
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Sec (U)  0   0.0%
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 +
(B)  0   0.0%
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(Z)  0   0.0%
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(X)  0   0.0%
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 +
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Total number of negatively charged residues (Asp + Glu): 19
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Total number of positively charged residues (Arg + Lys): 21
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Atomic composition:
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Carbon      C       785
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Hydrogen    H       1190
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Nitrogen    N       202
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Oxygen      O       212
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Sulfur      S         7
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Formula: C785H1190N202O212S7
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Total number of atoms: 2396
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Extinction coefficients:
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Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.
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Ext. coefficient    39085
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Abs 0.1% (=1 g/l)  2.289, assuming all pairs of Cys residues form cystines
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 +
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Ext. coefficient    38960
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Abs 0.1% (=1 g/l)  2.282, assuming all Cys residues are reduced
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Estimated half-life:
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The N-terminal of the sequence considered is M (Met).
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The estimated half-life is:
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                            30 hours (mammalian reticulocytes, in vitro).
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                            >20 hours (yeast, in vivo).
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                            >10 hours (Escherichia coli, in vivo).
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Instability index:
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The instability index (II) is computed to be 35.64
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This classifies the protein as stable.
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Aliphatic index: 70.00
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Grand average of hydropathicity (GRAVY): -0.385
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===Source===
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Chlamydomonas reinhardtii
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===References===
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 22:36, 27 September 2013

YCF54

Long Descriptions here

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 270




Amino acid sequence

MAPAAASADK ATAAEYYALV CNAEWFFMDP QNESVAEQLR EKVRFFKEQN KERDFFIVPN
PKWLDAKFPE QAKQVKRPCV ALVSTDKMWI TFMKLRLDRV LKIDLKSMPA SEVLAAGEAL
PDFKPDGKWT APYARYTPGW WNVFLPNH </F>


References and documentation are available. Please note the modified algorithm for extinction coefficient.

Number of amino acids: 148

Molecular weight: 17073.7

Theoretical pI: 8.50

Amino acid composition: Ala (A) 20 13.5% Arg (R) 7 4.7% Asn (N) 6 4.1% Asp (D) 9 6.1% Cys (C) 2 1.4% Gln (Q) 5 3.4% Glu (E) 10 6.8% Gly (G) 3 2.0% His (H) 1 0.7% Ile (I) 3 2.0% Leu (L) 11 7.4% Lys (K) 14 9.5% Met (M) 5 3.4% Phe (F) 10 6.8% Pro (P) 12 8.1% Ser (S) 5 3.4% Thr (T) 5 3.4% Trp (W) 6 4.1% Tyr (Y) 4 2.7% Val (V) 10 6.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% 

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19 Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon C 785 Hydrogen H 1190 Nitrogen N 202 Oxygen O 212 Sulfur S 7

Formula: C785H1190N202O212S7 Total number of atoms: 2396

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 39085 Abs 0.1% (=1 g/l) 2.289, assuming all pairs of Cys residues form cystines


Ext. coefficient 38960 Abs 0.1% (=1 g/l) 2.282, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 

                            30 hours (mammalian reticulocytes, in vitro).
                           >20 hours (yeast, in vivo).
                           >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.64 This classifies the protein as stable.


Aliphatic index: 70.00

Grand average of hydropathicity (GRAVY): -0.385

Source

Chlamydomonas reinhardtii

References