Difference between revisions of "Part:BBa K1080012"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1080012 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1080012 SequenceAndFeatures</partinfo> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <b>Amino acid sequence</b> | ||
+ | <FONT FACE="courier">MAMAASRQAV RVAAAVDADY RKREPKDVRV LVVGPTGYIG KFVVKELVSR GYNVVAFARE<br> NAGIKGKMGR EDIVKEFHGA EVRFGSVLDP ASLRDVAFKD PVDVVVSCLA SRTGGKKDSW<br> LIDYTATKNS LDVARASGAK HFVLLSAICV QKPLLEFQKA KLQFESDLQA AGDITYSIVR<br> PTAFFKSIAG QIDIVKKGNP YVMFGDGNLA ACKPISEADL ASFIADCVTE QNKVNKVLPI<br> GGPSKAFTAK QQADLLFNIT GLPPKYFPVP VALMDGMIGL FDSLAKLFPQ LEDSAEFARI<br> GKYYATESML VYDEARGVYR KTKRLVTART RWKTSSLVQ </FONT> | ||
+ | |||
+ | References and documentation are available. | ||
+ | Please note the modified algorithm for extinction coefficient. | ||
+ | |||
+ | -------------------------------------------------------------------------------- | ||
+ | Number of amino acids: 339 | ||
+ | |||
+ | Molecular weight: 37034.9 | ||
+ | |||
+ | Theoretical pI: 9.40 | ||
+ | |||
+ | Amino acid composition: Ala (A) 40 11.8% | ||
+ | Arg (R) 19 5.6% | ||
+ | Asn (N) 8 2.4% | ||
+ | Asp (D) 22 6.5% | ||
+ | Cys (C) 4 1.2% | ||
+ | Gln (Q) 11 3.2% | ||
+ | Glu (E) 14 4.1% | ||
+ | Gly (G) 24 7.1% | ||
+ | His (H) 2 0.6% | ||
+ | Ile (I) 16 4.7% | ||
+ | Leu (L) 27 8.0% | ||
+ | Lys (K) 29 8.6% | ||
+ | Met (M) 7 2.1% | ||
+ | Phe (F) 18 5.3% | ||
+ | Pro (P) 15 4.4% | ||
+ | Ser (S) 21 6.2% | ||
+ | Thr (T) 14 4.1% | ||
+ | Trp (W) 2 0.6% | ||
+ | Tyr (Y) 11 3.2% | ||
+ | Val (V) 35 10.3% | ||
+ | Pyl (O) 0 0.0% | ||
+ | Sec (U) 0 0.0% | ||
+ | |||
+ | (B) 0 0.0% | ||
+ | (Z) 0 0.0% | ||
+ | (X) 0 0.0% | ||
+ | |||
+ | |||
+ | Total number of negatively charged residues (Asp + Glu): 36 | ||
+ | Total number of positively charged residues (Arg + Lys): 48 | ||
+ | |||
+ | Atomic composition: | ||
+ | |||
+ | Carbon C 1670 | ||
+ | Hydrogen H 2668 | ||
+ | Nitrogen N 450 | ||
+ | Oxygen O 477 | ||
+ | Sulfur S 11 | ||
+ | |||
+ | Formula: C1670H2668N450O477S11 | ||
+ | Total number of atoms: 5276 | ||
+ | |||
+ | Extinction coefficients: | ||
+ | |||
+ | Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. | ||
+ | |||
+ | Ext. coefficient 27640 | ||
+ | Abs 0.1% (=1 g/l) 0.746, assuming all pairs of Cys residues form cystines | ||
+ | |||
+ | |||
+ | Ext. coefficient 27390 | ||
+ | Abs 0.1% (=1 g/l) 0.740, assuming all Cys residues are reduced | ||
+ | |||
+ | Estimated half-life: | ||
+ | |||
+ | The N-terminal of the sequence considered is M (Met). | ||
+ | |||
+ | The estimated half-life is: | ||
+ | 30 hours (mammalian reticulocytes, in vitro). | ||
+ | >20 hours (yeast, in vivo). | ||
+ | >10 hours (Escherichia coli, in vivo). | ||
+ | |||
+ | |||
+ | Instability index: | ||
+ | |||
+ | The instability index (II) is computed to be 31.02 | ||
+ | This classifies the protein as stable. | ||
+ | |||
+ | |||
+ | |||
+ | Aliphatic index: 91.21 | ||
+ | |||
+ | Grand average of hydropathicity (GRAVY): -0.019 | ||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | Chlamydomonas reinhardtii | ||
+ | |||
+ | ===References=== | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 22:35, 27 September 2013
DVR1
3,8-divinyl protochlorophyllidea 8-vinyl reductase - Predicted chloroplast transit peptide 1-58; [PMID: 15695432; 15849308]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 288
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Amino acid sequence
MAMAASRQAV RVAAAVDADY RKREPKDVRV LVVGPTGYIG KFVVKELVSR GYNVVAFARE
NAGIKGKMGR EDIVKEFHGA EVRFGSVLDP ASLRDVAFKD PVDVVVSCLA SRTGGKKDSW
LIDYTATKNS LDVARASGAK HFVLLSAICV QKPLLEFQKA KLQFESDLQA AGDITYSIVR
PTAFFKSIAG QIDIVKKGNP YVMFGDGNLA ACKPISEADL ASFIADCVTE QNKVNKVLPI
GGPSKAFTAK QQADLLFNIT GLPPKYFPVP VALMDGMIGL FDSLAKLFPQ LEDSAEFARI
GKYYATESML VYDEARGVYR KTKRLVTART RWKTSSLVQ
References and documentation are available. Please note the modified algorithm for extinction coefficient.
Number of amino acids: 339
Molecular weight: 37034.9
Theoretical pI: 9.40
Amino acid composition: Ala (A) 40 11.8% Arg (R) 19 5.6% Asn (N) 8 2.4% Asp (D) 22 6.5% Cys (C) 4 1.2% Gln (Q) 11 3.2% Glu (E) 14 4.1% Gly (G) 24 7.1% His (H) 2 0.6% Ile (I) 16 4.7% Leu (L) 27 8.0% Lys (K) 29 8.6% Met (M) 7 2.1% Phe (F) 18 5.3% Pro (P) 15 4.4% Ser (S) 21 6.2% Thr (T) 14 4.1% Trp (W) 2 0.6% Tyr (Y) 11 3.2% Val (V) 35 10.3% Pyl (O) 0 0.0% Sec (U) 0 0.0%
(B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1670 Hydrogen H 2668 Nitrogen N 450 Oxygen O 477 Sulfur S 11
Formula: C1670H2668N450O477S11 Total number of atoms: 5276
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 27640 Abs 0.1% (=1 g/l) 0.746, assuming all pairs of Cys residues form cystines
Ext. coefficient 27390
Abs 0.1% (=1 g/l) 0.740, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is:
30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.02 This classifies the protein as stable.
Aliphatic index: 91.21
Grand average of hydropathicity (GRAVY): -0.019
Source
Chlamydomonas reinhardtii