Difference between revisions of "Part:BBa K1092105"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1092105 short</partinfo> | <partinfo>BBa_K1092105 short</partinfo> | ||
| − | RFP | + | An RFP fragment adapted from the RFP part BBa_E1010, and its counterpart is the RFP C terminus (BBa_K1092106). It is adapted to be expressed downstream of a fusion protein such as our Voltage switch parts (BBa_K1092023 & BBa_K1092024). It is the N terminal of the RFP, and is capable of refolding with it’s counter part C terminal as demonstrated by the NYMU-Taipei 2010 iGEM team through the Biomolecular Fluorescent complementation (BiFC) to give fluorescent (BBa_K411103 & BBa_K411104). We adapt this protein to be the downstream of our voltage switch because we want it to become our effector of the switch. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 22:16, 27 September 2013
RFP N terminus
An RFP fragment adapted from the RFP part BBa_E1010, and its counterpart is the RFP C terminus (BBa_K1092106). It is adapted to be expressed downstream of a fusion protein such as our Voltage switch parts (BBa_K1092023 & BBa_K1092024). It is the N terminal of the RFP, and is capable of refolding with it’s counter part C terminal as demonstrated by the NYMU-Taipei 2010 iGEM team through the Biomolecular Fluorescent complementation (BiFC) to give fluorescent (BBa_K411103 & BBa_K411104). We adapt this protein to be the downstream of our voltage switch because we want it to become our effector of the switch.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]