Difference between revisions of "Part:BBa K1092024"

 
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<partinfo>BBa_K1092024 short</partinfo>
 
<partinfo>BBa_K1092024 short</partinfo>
  
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pSB1C3-T7-RBS-ssDsbA-PDZ Domain-Voltage Switch-RFP C terminus
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This is biobrick is the counterpart of the other biobrick T7-RBS-ssDsbA-PDZ Ligand-Voltage sensor peptide-RFP N terminus (BBa_K1092023), and is composed of the voltage switch (BBa_K1092009 & BBa_K1092010) and the RFP fragments (BBa_K1092105 & BBa_K1092106). In this Example, we demonstrate the possibility of using the voltage switch as a fast control of fluorescent signal. We made use of the biomolecular fluorescent complementation to achieve this purpose, and we worked on making the first reversible BiFC reaction in the world.
  
 
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Revision as of 22:08, 27 September 2013

T7-RBS-ssDsbA-PDZ Domain-Voltage Switch-RFP N terminus

This is biobrick is the counterpart of the other biobrick T7-RBS-ssDsbA-PDZ Ligand-Voltage sensor peptide-RFP N terminus (BBa_K1092023), and is composed of the voltage switch (BBa_K1092009 & BBa_K1092010) and the RFP fragments (BBa_K1092105 & BBa_K1092106). In this Example, we demonstrate the possibility of using the voltage switch as a fast control of fluorescent signal. We made use of the biomolecular fluorescent complementation to achieve this purpose, and we worked on making the first reversible BiFC reaction in the world.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 362