Difference between revisions of "Part:BBa K1100010"
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<partinfo>BBa_K1100010 short</partinfo> | <partinfo>BBa_K1100010 short</partinfo> | ||
| − | A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034), the Csy4 gene and the teminator (BBa_B0015). | + | A seious of parts are put together onto the pSB1C3 plasmid including the promter([https://parts.igem.org/Part:BBa_R0040 BBa_R0040]),csy4 insulator(16-nt), a RBS([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), the Csy4 gene and the teminator ([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]). |
The generator is designed to express the Csy4 for a futher usage for the cleavage to the csy4 insulator. | The generator is designed to express the Csy4 for a futher usage for the cleavage to the csy4 insulator. | ||
Meanwhile, it has a insulator itself to avoid the disturbance between the patterns. | Meanwhile, it has a insulator itself to avoid the disturbance between the patterns. | ||
Latest revision as of 22:06, 27 September 2013
Csy4 generator (with terminator)
A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034), the Csy4 gene and the teminator (BBa_B0015). The generator is designed to express the Csy4 for a futher usage for the cleavage to the csy4 insulator. Meanwhile, it has a insulator itself to avoid the disturbance between the patterns.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 448
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 164