Difference between revisions of "Part:BBa K1036005:Design"

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This part contains two subparts: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036004">BBa_K1036004</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D). <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036004">BBa_K1036004</a> digested by <i>Eco</i>R I & <i>Spe</i>I and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> digested by <i>Eco</i>R I & <i>Xba</i>I were ligated together by T4 ligase.
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This part contains two subparts: RBS-sfgfp(-LVA)-TT(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036004">BBa_K1036004</a>)and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D). RBS-sfgfp(-LVA)-TT digested by <i>Eco</i>R I & <i>Spe</i>I and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> digested by <i>Eco</i>R I & <i>Xba</i>I were ligated together by T4 ligase.
 
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Latest revision as of 21:58, 27 September 2013


sfgfp (with LVA-tag) under plux R control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914
    Illegal BsaI.rc site found at 2017
    Illegal SapI.rc site found at 31


Design Notes

This part contains two subparts: RBS-sfgfp(-LVA)-TT(BBa_K1036004)and BBa_K546000 (located in 2013-Plate 1-12D). RBS-sfgfp(-LVA)-TT digested by EcoR I & SpeI and BBa_K546000 digested by EcoR I & XbaI were ligated together by T4 ligase.

Source

The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.

References

1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)
3. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)