Difference between revisions of "Part:BBa K1036006:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | + | This part contains two subparts: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_F2621">BBa_F2621</a> (located in 2012-Plate 2-21F) and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036005">BBa_K1036005</a>.<br/><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_F2621">BBa_F2621</a> digested by <i>Eco</i>R & <i>Spe</i>I and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036005">BBa_K1036005</a> digested by <i>Eco</i>R & <i>Xba</i>I were ligated together by T4 ligase. | |
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===Source=== | ===Source=== | ||
Latest revision as of 21:57, 27 September 2013
lux pL controlled luxR with lux pR controlled sfgfp (with LVA-tag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3903
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Illegal BsaI.rc site found at 2080
Illegal BsaI.rc site found at 3183
Illegal SapI.rc site found at 1197
Design Notes
This part contains two subparts: BBa_F2621 (located in 2012-Plate 2-21F) and BBa_K1036005.
BBa_F2621 digested by EcoR & SpeI and BBa_K1036005 digested by EcoR & XbaI were ligated together by T4 ligase.
Source
The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.
References
1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)
3. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)