Difference between revisions of "Part:BBa K1036004:Design"

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The <i>sfgfp</i> gene was amplifed from the biobrick pSB1A3-RBS-sfgfp-TT provided by Peking Unversity via PCR and sequenced after TA-cloning. Then RBS(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">BBa_B0034</a>), LVA-tag and terminator(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>) were fused with <i>ndh</i> via fusion PCR becoming RBS-<i>ndh</i>-LVA-TT marked as <i><b>ndh</b></i>. The DH5α colony carried <i>ndh</i> gene was sequenced after TA-cloning.
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The <i>sfgfp</i> gene with RBS (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034">BBa_B0034</a>) was amplifed from the biobrick pSB1A3-RBS-sfgfp-TT provided by Peking Unversity via PCR. The LVA-tag and terminator(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>) were fused with <i>sfgfp</i> via fusion PCR. The DH5α colony carried <i>sfgfp</i> gene was sequenced after TA-cloning.
 
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Revision as of 21:51, 27 September 2013


sfgfp with LVA-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

The sfgfp gene with RBS (BBa_B0034) was amplifed from the biobrick pSB1A3-RBS-sfgfp-TT provided by Peking Unversity via PCR. The LVA-tag and terminator(BBa_B0015) were fused with sfgfp via fusion PCR. The DH5α colony carried sfgfp gene was sequenced after TA-cloning.

Source

The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.

References

1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)