Difference between revisions of "Part:BBa K1064002"
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| − | + | We use GFP generator (BBa_E0840) under the control pSOS promotor (BBa_J22106) to detect DNA damage. Our team assemble both of part becoming a new part (BBa_K1064002). pSOS promotor is a promotor that regulation SOS response, this promotor will active when LexA (a protein repressor in operator) cleavage by RecA protein. When DNA was damage, Rec A protein | |
| − | + | will active by binding with ssDNA that produce when replication stop in DNA damage site. The active RecA protein will cleavage LexA (a dimer protein) that binding in operator site, this phenomenon made promotor SOS will expression the gene under its regulation. The strong RBS on BBa_E0840 allow the expression of GFP occurs after the DNA damage expossure. Here, our team test this part toward the DNA damage caused by UV irradiation and found the green fluorescence using fluorescence microscopy [[File:PSOSGC_10_detik_(fluoresens).jpg]], while the control (without UV irradiation) doesn't show the green fluorescence [[File:Kontrol_pSOSGC_(fluoresesns).jpg]] | |
| − | (BBa_J22106). Our team assemble both of part becoming a new part (BBa_K1064002). pSOS | + | |
| − | + | ||
| − | promotor is a promotor that regulation SOS response, this promotor will active when LexA (a | + | |
| − | + | ||
| − | protein repressor in operator) cleavage by RecA protein. When DNA was damage, Rec A protein | + | |
| − | + | ||
| − | will active by binding with ssDNA that produce when replication stop in DNA damage site. | + | |
| − | + | ||
| − | The active RecA protein will cleavage LexA (a dimer protein) that binding in operator site, this | + | |
| − | + | ||
| − | phenomenon made promotor SOS will expression the gene under its regulation. | + | |
Revision as of 21:48, 27 September 2013
We use GFP generator (BBa_E0840) under the control pSOS promotor (BBa_J22106) to detect DNA damage. Our team assemble both of part becoming a new part (BBa_K1064002). pSOS promotor is a promotor that regulation SOS response, this promotor will active when LexA (a protein repressor in operator) cleavage by RecA protein. When DNA was damage, Rec A protein
will active by binding with ssDNA that produce when replication stop in DNA damage site. The active RecA protein will cleavage LexA (a dimer protein) that binding in operator site, this phenomenon made promotor SOS will expression the gene under its regulation. The strong RBS on BBa_E0840 allow the expression of GFP occurs after the DNA damage expossure. Here, our team test this part toward the DNA damage caused by UV irradiation and found the green fluorescence using fluorescence microscopy .jpg)
, while the control (without UV irradiation) doesn't show the green fluorescence .jpg)