Difference between revisions of "Part:BBa K1166001"

Revision as of 21:46, 27 September 2013

Fumarate-Nitrate Reductase

FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under anoxic conditions FNR is in the active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008). Therefore, expression of FNR when hypoxic promoters are used would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.

References

Reinhart F, Achebach S, Koch T, Unden G. (2008). Reduced apo-fumarate nitrate reductase regulator (apoFNR) as the major form of FNR in aerobically growing Escherichia coli. J Bacteriol. 190(3):879-86.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under anoxic conditions FNR is in the active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008).
-Therefore expression of FNR when hypoxic promoters are used would help to have a more sensitive hypoxic response.  Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.
+Therefore, expression of FNR when hypoxic promoters are used would help to have a more sensitive hypoxic response.  Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.
 ==References==