Difference between revisions of "Part:BBa K1051702"
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| − | ===Usage and Biology=== | + | ===Usage and Biology===--> |
| + | <p>SRC1 intron has two 5' splicing site whose efficiency is regulated by protein hub1. Originally, the existence of the intron would produce two mature mRNA in proportion. After engineering, the existence of protein hub1 regulates to the preservation of intron precisely, which would make the following mRNA be expressed normally or not. As for the silencing of wild type gene HUB1, we choose CRISPRi that is comparably easy to use reversibly.</p> | ||
| + | https://static.igem.org/mediawiki/parts/9/96/SICI_principle.jpg | ||
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Latest revision as of 21:43, 27 September 2013
SRC1 Intron+CGG
Intron from SRC1 with additional CGG at 3' Can be spliced into two different forms(5'L or 5'S) by regulating the presence of Hub1 or Bud31
SRC1 intron has two 5' splicing site whose efficiency is regulated by protein hub1. Originally, the existence of the intron would produce two mature mRNA in proportion. After engineering, the existence of protein hub1 regulates to the preservation of intron precisely, which would make the following mRNA be expressed normally or not. As for the silencing of wild type gene HUB1, we choose CRISPRi that is comparably easy to use reversibly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]