Difference between revisions of "Part:BBa K1051700"
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can be spliced into two different forms by regulating the presence of Hub1 or Bud31 | can be spliced into two different forms by regulating the presence of Hub1 or Bud31 | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
<p>SRC1 intron has two 5' splicing site whose efficiency is regulated by protein hub1. Originally, the existence of the intron would produce two mature mRNA in proportion. After engineering, the existence of protein hub1 regulates to the preservation of intron precisely, which would make the following mRNA be expressed normally or not. As for the silencing of wild type gene HUB1, we choose CRISPRi that is comparably easy to use reversibly.</p> | <p>SRC1 intron has two 5' splicing site whose efficiency is regulated by protein hub1. Originally, the existence of the intron would produce two mature mRNA in proportion. After engineering, the existence of protein hub1 regulates to the preservation of intron precisely, which would make the following mRNA be expressed normally or not. As for the silencing of wild type gene HUB1, we choose CRISPRi that is comparably easy to use reversibly.</p> | ||
https://static.igem.org/mediawiki/parts/9/96/SICI_principle.jpg | https://static.igem.org/mediawiki/parts/9/96/SICI_principle.jpg | ||
Revision as of 21:42, 27 September 2013
SRC1 Intron -4bp at 5
Intron from SRC1 can be spliced into two different forms by regulating the presence of Hub1 or Bud31
SRC1 intron has two 5' splicing site whose efficiency is regulated by protein hub1. Originally, the existence of the intron would produce two mature mRNA in proportion. After engineering, the existence of protein hub1 regulates to the preservation of intron precisely, which would make the following mRNA be expressed normally or not. As for the silencing of wild type gene HUB1, we choose CRISPRi that is comparably easy to use reversibly.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]