Difference between revisions of "Part:BBa K1054021"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1054021 short</partinfo>
 
<partinfo>BBa_K1054021 short</partinfo>
  
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ssDsbA+beta-lactamase+lgt+streptavidin
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This part is used to confirm the localization of membrane scaffold. Beta-lactamase is fused to the periplasmic end of membrane scaffold. If the fusion protein is successfully targeted to the inner-membrane, the bacteria will have ampicillin resistance.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:40, 27 September 2013

ssDsbA+beta-lactamase+lgt+streptavidin

This part is used to confirm the localization of membrane scaffold. Beta-lactamase is fused to the periplasmic end of membrane scaffold. If the fusion protein is successfully targeted to the inner-membrane, the bacteria will have ampicillin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1862
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1664
    Illegal AgeI site found at 1904
    Illegal AgeI site found at 1955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 723
    Illegal BsaI site found at 1285