Difference between revisions of "Part:BBa K1124011:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1124011 short</partinfo>
 
<partinfo>BBa_K1124011 short</partinfo>
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===Design Notes===
 
===Design Notes===
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PCR
 
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 +
This part was cloned from ''E. coli'' BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.
  
 
===Source===
 
===Source===
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''E. coli'' BL21(DE3)
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 +
'''Links to NCBI gene detabase'''
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 +
http://www.ncbi.nlm.nih.gov/gene/8183130 (hpaC)
  
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PCR <em>E .coli</em> BL21
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http://www.ncbi.nlm.nih.gov/gene/8183129 (hpaB)
  
 
===References===
 
===References===

Revision as of 21:39, 27 September 2013

hpaBC (+RBS(natural))


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1877


Design Notes

This part was cloned from E. coli BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.

Source

E. coli BL21(DE3)

Links to NCBI gene detabase

http://www.ncbi.nlm.nih.gov/gene/8183130 (hpaC)

http://www.ncbi.nlm.nih.gov/gene/8183129 (hpaB)

References