Difference between revisions of "Part:BBa K1161000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 21:33, 27 September 2013
violacein A coding sequence
Violacein A (Vio)A is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1033
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]