Difference between revisions of "Part:BBa K1054008"

Latest revision as of 21:32, 27 September 2013

pLAC promotor,atrazine-inducible Riboswitch, GFP+Terminator

This is the part used to standardize the atrazine Riboswitch.

The protocol is as follows: 1. Cultivate the BL21 containing BBa_ in LB at 37℃180rpm until OD600 reaches 0.5.

2. Induce the bacteria with 1mM IPTG (final concentration), and cultivate them at 37℃180rpm for 4hrs.

3. Dilute the suspension culture with LB to 0.1OD600 . Theophylline is added at concentration ranging from 1.00E-06 mol/L to1.00E-01 mol/L.

4. Measure the RFU ( ex488/em525) and OD600 synchronously.

5. Data Analysis

Graphic Model about how RFU/OD changes at different time and different concentration of Atrzine.

Graphic Model about how GFP Change Rate changes at different time and different concentration of Atrzine.

Sequence and Features

Assembly Compatibility:

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1054008 short</partinfo>
 This is the part used to standardize the atrazine Riboswitch.
+The protocol is as follows:
+. Cultivate the BL21 containing BBa_ in LB at 37℃180rpm until OD600 reaches 0.5.
+. Induce the bacteria with 1mM IPTG (final concentration), and cultivate them at 37℃180rpm for 4hrs.
+. Dilute the suspension culture with LB to 0.1OD600 . Theophylline is added at concentration ranging from 1.00E-06 mol/L to1.00E-01 mol/L.
+. Measure the RFU ( ex488/em525) and OD600 synchronously.
+. Data Analysis
+[[File:500px-ZJU-Standardization 2.1.jpg]]
+Graphic Model about how RFU/OD changes at different time and different concentration of Atrzine.
+[[File:500px-ZJU-Standardization 2.2.jpg]]
+Graphic Model about how GFP Change Rate changes at different time and different concentration of Atrzine.
 <!-- Add more about the biology of this part here