Difference between revisions of "Part:BBa K1031011"
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== '''Components and Characterization''' == | == '''Components and Characterization''' == | ||
| − | K1031011 consists of RBS B0034[https://parts.igem.org/Part:BBa_B0034], ribozyme RiboJ and transcription factor φR73 delta. φR73 functions as an activator of | + | K1031011 consists of RBS B0034[https://parts.igem.org/Part:BBa_B0034], ribozyme RiboJ and transcription factor φR73 delta. φR73 functions as an activator of <html><a href="https://parts.igem.org/Part:BBa_K1031021">hybrid promoter</a></html>. It is the activation node of the Band-pass Filter circuit ('''Fig 1'''). |
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| − | <img src="https://static.igem.org/mediawiki/igem.org/thumb/3/33/Peking2013_part_K1031011.png/800px-Peking2013_part_K1031011.png" | + | <img src="https://static.igem.org/mediawiki/igem.org/thumb/3/33/Peking2013_part_K1031011.png/800px-Peking2013_part_K1031011.png" style="width:700px; margin-left:170px" /> |
| + | <p style="text-align center"><b>Fig 1</b> Activation circuit of Band-pass Filter. The green oval represents RBS B0034. Ribozyme RiboJ is marked in dark blue. φR73 coding sequence is shown as square with dark red. φR73 activates hybrid promoter, which is marked with red and blue, via binding upstream activating sequence. Then the expression of sfGFP is enhanced. | ||
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== '''Data shown''' == | == '''Data shown''' == | ||
| − | Previous work done by Dr. Lou Chunbo showed φR73 had obvious activating effect to downstream promoter. Molecular cloning which connected φR73 with Psal promoter enables the circuit to response to salicylic acid ('''Fig 2'''). | + | Previous work done by Dr. Lou Chunbo showed φR73 had obvious activating effect to downstream promoter. Molecular cloning which connected φR73 with ''Psal'' promoter enables the circuit to response to salicylic acid ('''Fig 2'''). |
<html> | <html> | ||
| − | <img src="https://static.igem.org/mediawiki/igem.org/thumb/8/84/Peking2013_part_PhiR_73.jpg/800px-Peking2013_part_PhiR_73.jpg" | + | <img src="https://static.igem.org/mediawiki/igem.org/thumb/8/84/Peking2013_part_PhiR_73.jpg/800px-Peking2013_part_PhiR_73.jpg" style="width:600px; margin-left:170px" /> |
| + | <p style="text-align center"><b>Fig 2</b> Induction ratio of activation circuit when exposed to inducers. Horizontal axis represents salicylic acid at different concentration. Vertical axis stands for induction ratio calculated from fluorescence intensity. (Data from Dr.Lou Chunbo) | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 20:49, 27 September 2013
B0034-RiboJ-φR73
Components and Characterization
K1031011 consists of RBS B0034[1], ribozyme RiboJ and transcription factor φR73 delta. φR73 functions as an activator of hybrid promoter. It is the activation node of the Band-pass Filter circuit (Fig 1).
Fig 1 Activation circuit of Band-pass Filter. The green oval represents RBS B0034. Ribozyme RiboJ is marked in dark blue. φR73 coding sequence is shown as square with dark red. φR73 activates hybrid promoter, which is marked with red and blue, via binding upstream activating sequence. Then the expression of sfGFP is enhanced.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Data shown
Previous work done by Dr. Lou Chunbo showed φR73 had obvious activating effect to downstream promoter. Molecular cloning which connected φR73 with Psal promoter enables the circuit to response to salicylic acid (Fig 2).
Fig 2 Induction ratio of activation circuit when exposed to inducers. Horizontal axis represents salicylic acid at different concentration. Vertical axis stands for induction ratio calculated from fluorescence intensity. (Data from Dr.Lou Chunbo)