Difference between revisions of "Part:BBa J31000:Design"

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<font color='blue'>GAATTC</font color><font color='brown'>GCGGCCGC</font color><font color='green'>T</font color><font color='orange'>TCTAGA</font color><font color='red'>-</font color><u>ATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGACCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAAAGTATGTAAATAAAGGCGATACTCTTGTCGTCTGGAAATTAGACAGACTGGGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACGTGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGATGGGGCGATTCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCGCCCTCGGGCGATCAACAAACATGAACAGGAACAGATTAGTCGGCTATTAGAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATTAA</u><font color='tan'>-</font color><font color='lime green'>ACTAGT</font color><font color='navy'>-</font color><font color='pink'>GCGGCCGC</font color><font color='cyan'>CTGCAG</font color>
 
<font color='blue'>GAATTC</font color><font color='brown'>GCGGCCGC</font color><font color='green'>T</font color><font color='orange'>TCTAGA</font color><font color='red'>-</font color><u>ATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGACCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAAAGTATGTAAATAAAGGCGATACTCTTGTCGTCTGGAAATTAGACAGACTGGGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACGTGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGATGGGGCGATTCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCGCCCTCGGGCGATCAACAAACATGAACAGGAACAGATTAGTCGGCTATTAGAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATTAA</u><font color='tan'>-</font color><font color='lime green'>ACTAGT</font color><font color='navy'>-</font color><font color='pink'>GCGGCCGC</font color><font color='cyan'>CTGCAG</font color>
 +
 +
There is no <font color='red'>G</font color> spacer between the <font color='orange'>XbaI</font color> and the <u>insert</u>.
 +
 +
There is no <font color='tan'>T</font color> spacer between the <u>insert</u> and the <font color='lime green'>SpeI</font color> site.
 +
 +
There is no <font color='navy'>A</font color> spacer between the <font color='lime green'>SpeI</font color> site and the <font color='pink'>NotI site</font color>.
 +
 +
The last C of the <font color='pink'>NotI </font color>site is not conserved with the initial C from the <font color='cyan'>PstI site</font color>.
 +
 +
The BB suffix currently has this sequence for <font color='pink'>NotI</font color> and <font color='cyan'>PstI</font color>:
 +
<font color='pink'>GCGGCCGc</font color><font color='cyan'>CTGCAG</font color>
 +
 +
But it should have been:
 +
<font color='pink'>GCGGCCG</font color><font color='purple'>C</font color><font color='cyan'>TGCAG</font color>
 +
 +
There is no <font color='navy'>spacer</font color> between the <font color='pink'>NotI</font color> site and the <font color='lime green'>SpeI</font color> site. There should be a T.
  
 
===Source===
 
===Source===

Revision as of 20:15, 26 July 2006


DNA-invertase Hin from Salmonella typhimurium


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is cloned into plasmid pSB1A2.

Biobricks

The Biobricks attached to this part are not correct.

GAATTCGCGGCCGCTTCTAGA-ATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGACCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAAAGTATGTAAATAAAGGCGATACTCTTGTCGTCTGGAAATTAGACAGACTGGGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACGTGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGATGGGGCGATTCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCGCCCTCGGGCGATCAACAAACATGAACAGGAACAGATTAGTCGGCTATTAGAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATTAA-ACTAGT-GCGGCCGCCTGCAG

There is no G spacer between the XbaI and the insert.

There is no T spacer between the insert and the SpeI site.

There is no A spacer between the SpeI site and the NotI site.

The last C of the NotI site is not conserved with the initial C from the PstI site.

The BB suffix currently has this sequence for NotI and PstI: GCGGCCGcCTGCAG

But it should have been: GCGGCCGCTGCAG

There is no spacer between the NotI site and the SpeI site. There should be a T.

Source

Salmonella typhimurium

References