Difference between revisions of "Part:BBa K157004"

Revision as of 19:40, 27 September 2013

Fluoresceine-A-binding

[http://2013.igem.org/Team:TU-Munich Team TU Munich 2013] privided a higher engineered variant of this anticalin, which is also compatible to RFC10 and RFC25 (see: BBa_K1159003) and has a higher affinity to fluorescein
(--TU Munich 19:30, 27 September 2013 (UTC))

Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from Pieris brassicae, originally designed by Arne Skerra [see Ref. 1,2].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 248
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K157004 short</partinfo>
-'''''Note: There is a improved (triple mutant version) of this Biobrick with higher binding affinity for Fluorescein; this mutations are also designed and introduced by Skerra lab: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1159003 BBa_K1159003]'''''
+[http://2013.igem.org/Team:TU-Munich Team TU Munich 2013] privided a higher engineered variant of this anticalin, which is also compatible to RFC10 and RFC25 (see: <partinfo>BBa_K1159003</partinfo>) and has a higher affinity to fluorescein  <br>
+(<small>--[[User:TU_Munich|TU Munich]] 19:30, 27 September 2013 (UTC)</small>)
 Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from ''Pieris brassicae'', originally designed by Arne Skerra [see Ref. 1,2].