Difference between revisions of "Part:BBa K1124009"

Revision as of 19:34, 27 September 2013

anti-tyrR sRNA

Usage and Biology

The Effects on tyrosine metabolism of TyrR TyrR is a transcriptional regulator which represses the expression of several enzymes involved in the tyrosine producing pathway in E. coli. It was shown that TyrR repression leaded to increase of the tyrosine production.

RNA Silencing Utilizing MicC sRNA Scaffold

The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold(BBa_K1124005) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold(BBa_K1124005)). In this part,anti-tyrR sRNA, the target-binding sequence is complementary to the first 24bp of tyrR CDS(that is, the sequence from start codon ATG and the following 7 triplet codons). We expect that this part will repress tyrR expression in post-transcriptional way and therefore will increase tyrosine production. With this part, we constructed tyrosine synthesis device(BBa_K1124106), which can likely increase tyrosine production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-'''The Effects on Hydrogen Production of HycA'''
+'''The Effects on tyrosine metabolism of TyrR'''
+TyrR is a transcriptional regulator which represses the expression of several enzymes involved in the tyrosine producing pathway in ''E. coli''.
-Hydrogen production in ''Escherichia coli'' is performed by Formate Hydrogen Lyase complex(FHL complex), which catabolizes formate into hydrogen and carbon dioxide. FHL complex consists of proteins named HycB, HycC, HycD, HycE, HycF, HycG, and FdhF. Most of these proteins other than FdhF are coded in ''hyc'' operon, which is transcriptionally activated and inhibited by FhlA([[Part:BBa K778001]], [[Part:BBa K778002]]) and HycA, respectively. It is known that''fhlA'' overexpression increases hydrogen production in ''E. coli'', so we expects that repression of ''hycA'' expression will also lead to hydrogen production increasing.
+It was shown that TyrR repression leaded to increase of the tyrosine production.
 '''RNA Silencing Utilizing MicC sRNA Scaffold'''
-The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold([[BBa_K1124005]]) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold([[BBa_K1124005]])). In this part,anti-hycA sRNA, the target-binding sequence is complementary to the first 24bp of hycA CDS(that is, the sequence from start codon ATG and the following 7 triplet codons).
+The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold([[BBa_K1124005]]) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold([[BBa_K1124005]])). In this part,anti-tyrR sRNA, the target-binding sequence is complementary to the first 24bp of tyrR CDS(that is, the sequence from start codon ATG and the following 7 triplet codons).
-We expect that this part will repress ''hycA'' expression in post-transcriptional way, and that expressing this part under proper promoters may lead to hydrogen production increase.
+We expect that this part will repress ''tyrR'' expression in post-transcriptional way and therefore will increase tyrosine production.
+With this part, we constructed tyrosine synthesis device([[BBa_K1124106]]), which can likely increase tyrosine production.
 <span class='h3bb'>Sequence and Features</span>