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| − | ====37°C RBS====
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| − | <p>Using biobrick, P<sub>cons</sub> + 37°C RBS + mGFP+J61048, we tested the function of the 37°C RBS at room temperature (around 25°C) and at 37°C.</p>
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| − | [[File:NCTU_37RBS_biobrick.png|center|400px|Figure 1. The biobrick for testing the function of 37°C RBS.]]
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| − | [[File:NCTU_Test_37RBS_fluo_pic.png|center|600px|Figure 2. The differences between the level of GFP expressions can be easily observed under UV light.]]
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| − | [[File:NCTU_Test_37RBS_ependorf.png|center|600px|Figure 3. The differences between the level of GFP expressions.]]
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| − | As shown in Figure 4 the normalized expression, obtained by dividing the florescence expression (emmision 612 nm and excitation 584 nm) with the OD<sub>600</sub> value, of GFP under 37°C is much higher than the expression under room temperature. Such result demonstrates the fact that 37°C RBS can effectively regulate gene expression by responding to temperature. The increased kinetic energy at 37°C is sufficient to cause the 37°C RBS to unfold and become available for ribosome binding. At room temperature, however, there isn't sufficient kinetic energy to unfold the hairpin structure and the structure is preserved. As a result, the translational efficiency is very low at room tempersture.
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| − | [[File:NCTU_Test_functional_test_of_37RBS.png|center|600px|Figure 4. The normalized expression (florescence expression/ OD value) under 37°C is higher than the expression under room temperature by about 6 folds.]]
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| − | </div></div>
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