Difference between revisions of "Part:BBa K1078000:Design"
Andresochoa (Talk | contribs) (→Design Notes) |
Andresochoa (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | After synthesis, in order to build our sensor the part was introduced in pPIC9K plasmid to allow its genomic recombination into the Pichia pastoris genome, substituting the original Mxr1 found in the yeast. The sequenced was modified to remove restriction sites, making it compatible to RFC10 | + | After synthesis, in order to build our sensor the part was introduced in pPIC9K plasmid to allow its genomic recombination into the ''Pichia pastoris'' genome, substituting the original Mxr1 found in the yeast. The sequenced was modified to remove restriction sites, making it compatible to RFC10 |
===Source=== | ===Source=== | ||
Latest revision as of 18:37, 27 September 2013
Mxr1 (methanol expression regulator 1) modified
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 240
Illegal XhoI site found at 137 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 430
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 179
Illegal SapI.rc site found at 635
Design Notes
After synthesis, in order to build our sensor the part was introduced in pPIC9K plasmid to allow its genomic recombination into the Pichia pastoris genome, substituting the original Mxr1 found in the yeast. The sequenced was modified to remove restriction sites, making it compatible to RFC10
Source
The part was obtained by synthesis.
References
The modified Mxr1 sequence was found in Parua PK, Ryan PM, Trang K, Young ET: Pichia pastoris 14-3-3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction. Mol Microbiol 2012, 85:282-298.