Difference between revisions of "Part:BBa K1051800"

Revision as of 18:31, 27 September 2013

protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

     Protein dCas9 is the key of the technology CRISPRi,and this part is the gene

of the dcas9. Deleting its ending codon is to provide a convenient way to add other
protein domain to improve the function of dCas9.

      
      So this part is used with sgRNA(k1051801,k1051802).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2740
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2886
Illegal BsaI site found at 3740
Illegal BsaI.rc site found at 1411

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1051800 short</partinfo>
-       Protein dCas9 is the key of the technology CRISPRi,and this part is the gene of the dcas9. Deleting its ending codon is to provide a convenient way to add other protein domain to improve
+       Protein dCas9 is the key of the technology CRISPRi,and this part is the gene<br/>
-the function of dCas9.
+of the dcas9. Deleting its ending codon is to provide a convenient way to add other<br/>
+protein domain to improve the function of dCas9.
 <!-- Add more about the biology of this part here -->
         https://static.igem.org/mediawiki/parts/1/13/CRISPRi_principle.png
-        So this part is used with sgRNA(k1051801,k1051802)
+        So this part is used with sgRNA(k1051801,k1051802).
 ===Usage and Biology===