Difference between revisions of "Part:BBa K1084121:Design"
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===Design Notes=== | ===Design Notes=== | ||
This part is constructed to measure translation efficiency of SD2 (BBa_K1084101). | This part is constructed to measure translation efficiency of SD2 (BBa_K1084101). | ||
| − | + | We used LacZα (BBa_I732006) as reporter gene and performed β-Galactosidase assay. | |
Latest revision as of 17:33, 27 September 2013
pTet SD2 LacZα dT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is constructed to measure translation efficiency of SD2 (BBa_K1084101). We used LacZα (BBa_I732006) as reporter gene and performed β-Galactosidase assay.
Source
Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.
References
V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology