Difference between revisions of "Part:BBa K1024005"

Revision as of 16:29, 27 September 2013

Report yeast (inducible ADE2)

Part Name: BBa_K1024005

Short Description: Report yeast (inducible ADE2)

Part Type: Signaling

Design: The part was the reporter of the pathogen detector. Tet operator and CYC1 TATA region was followed by ADE2 gene. In the yeast S. cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Mated with sensor yeast (BBa_K1024004) which is induced by AHL, the ADE2 gene will be activated and rescuing the ADE2 knockout yeast by making it white.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1053
Illegal BamHI site found at 456
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1024005 short</partinfo>
-<img height="auto" src="https://static.igem.org/mediawiki/2013/c/c7/Tsinghua_BBa_K1024000.png" width="100%"/>
 <p>
 					<b>Part Name:</b> BBa_K1024005
@@ Line 15: / Line 15: @@
 					<b>Design:</b> The part was the reporter of the pathogen detector. Tet operator and CYC1 TATA region was followed by ADE2 gene. In the yeast S. cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Mated with sensor yeast (BBa_K1024004) which is induced by AHL, the ADE2 gene will be activated and rescuing the ADE2 knockout yeast by making it white.
 				</p>
+https://static.igem.org/mediawiki/igem.org/a/a5/Tsinghua-BB-005.png