Difference between revisions of "Part:BBa K1024005"
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Revision as of 16:28, 27 September 2013
Report yeast (inducible ADE2)
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Part Name: BBa_K1024005
Short Description: Report yeast (inducible ADE2)
Part Type: Signaling
Design: The part was the reporter of the pathogen detector. Tet operator and CYC1 TATA region was followed by ADE2 gene. In the yeast S. cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Mated with sensor yeast (BBa_K1024004) which is induced by AHL, the ADE2 gene will be activated and rescuing the ADE2 knockout yeast by making it white.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1053
Illegal BamHI site found at 456 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]