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===Usage and Biology===

Latest revision as of 16:26, 27 September 2013

cln2 promoter (G1 phase to S phase)

Purpose

Works for the cell synchronization device, activates Cdc28p kinase to promote the G1 to S phase transition

Principle

"CLn2" is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .
Experimental results show that it activates Cdc28p kinase to promote the G1 to S phase transition .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 511