Difference between revisions of "Part:BBa K1051300"

Revision as of 16:24, 27 September 2013

cln2 promoter (G1 phase to S phase)

Purpose

Works for the cell synchronization device, activates Cdc28p kinase to promote the G1 to S phase transition

Principle

"CLn2" is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .
Experimental results show that it activates Cdc28p kinase to promote the G1 to S phase transition .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 511

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1051300 short</partinfo>
+<h3>Purpose</h3>
-It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the  metabolic product of the Bacterial you use for transformation .
+Works for the cell synchronization device, activates Cdc28p kinase to promote the G1 to S phase transition
+<h3>Principle</h3>
+"CLn2" is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the  metabolic product of the Bacterial you use for transformation .<br />
+Experimental results show that it activates Cdc28p kinase to promote the G1 to S phase transition .
 <!-- Add more about the biology of this part here