Difference between revisions of "Part:BBa K1051200"

 
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<partinfo>BBa_K1051200 short</partinfo>
 
<partinfo>BBa_K1051200 short</partinfo>
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<h3>Purpose</h3>
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Works for the degradation of fluorescent proteins we used.
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<br />
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<h3>Principle</h3>
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'''Ubiquitin''',a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).
  
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ubiquitin
 
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 16:23, 27 September 2013

Ubiquitin

Purpose

Works for the degradation of fluorescent proteins we used.

Principle

Ubiquitin,a small regulatory protein that has been found in almost all tissues of eukaryotic organisms,Ubiquitination is a post translational modification where ubuiquitin is attached to a substrate protein.It is carried out in three main steps; activation, conjugation and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]